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Title   자궁경부암 세포주의 HPV - URR 변이 상태에 따른 Interferon/Retinoic Acid 의 효과 분석 ( The Effects of Interferon / Retinoic Acid on Cervical Cancer Cell Lines According to the Mutational Status of HPV - URR )
Publicationinfo   2000 Jan; 032(05): 884-895.
Key_word   Humanpapillomavirus ( HPV ), Mutation, Retinoic acid, IFN-γ
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Abstract   Purpose: We investigated the effects of all-trans-retinoic acid (ATRA) and/or interferon- y (IFN- y) on the growth of various cervical cancer cell lines and HPV E6/E7 expression. The relationships between the functional activities of HPV-URR and the growth inhibition were identified. Materials and Methods: Four groups of cell lines were included; i) with integrated form of HPV-16 DNA (SNU-17, CaSki), ii) episomal form of HPV-16 (SNU-523), iii) integrated form of HPV-l8 (SNU-1160, HeLa) and iv) episomal form of HPV-18 (SNU-1245). The promoter activity of HPV-URR was confirmed by transient transfection assay in C33A using the HPV-18 URR-CAT reporter plasmid. Results: Selective mutation was detected in TEF-1 (transcriptional enhancer factor) binding site in SNU-17, and the activity of URR in SNU-17 was higher than that of the prototype. The proliferation was more inhibited in SNU-17 by IFN- y (10 ng/ml) than in SNU-902, CaSki and HeLa. The increase of the HPV-URR activity might play a role in the inhibition of growth by interferon-g. The expression of HPV-16 E6/E7 were significantly decreased by ATRA or 1FN- y. Conclusion: Point mutation at TEF-1 binding site of SNU-17 was related with the increased transcriptional activity of URR. Mutation in the HPV-URR and alteration of HPV-URR activity in SNU-17 might be related with significant growth suppression by IFN- z.
저 자   남궁성은(Sung Eun Namkoong),엄수종(Soo Jong Um),박종섭(Jong Sup Park),김태연(Tae Yeon Kim),김찬주(Chan Joo Kim),김은주(Eun Joo Kim),박태철(Tae Chul Park),박재갑(Jae Gahb Park)